日本 Jaica老化制御研究所代理
- 日本老化制御研究所自成立以来就对抗衰老进行研究，它是在有助于健康促进的理念下创建的。结果，此研究所在开发DNA氧化损伤测试试剂盒方面取得了很大的成功，并在该领域处于了领先地位。
本研究所现在销售许多氧化应激相关产品，这些产品可应用于医学，药学，生化学，食品科学等。它还利用其独特的技术开展检测服务。随着社会老龄化的发展，预防医学与抗衰老引起了越来越多的关注，在这些领域中，我们研究所将会通过开发和提供研究产品来助于营造一个健康，长寿的社会。
产品类型：
1. DNA氧化检测产品；
2. 蛋白氧化检测产品；
3. 脂肪氧化检测产品；
4. 抗氧化检测产品。
特性：
1. 高专一性、高灵敏性；
2. 不与RNA损伤产生交叉反应；
3. 易于操作、简单快速；
4. 无需昂贵的设备和样品预处理。
The Japan Institute for the Control of Aging has conducted research into control of aging since its foundation under the doctrine of contributing health promotion. As a result, the institute succeeded in developing a DNA oxidative damage measuring kit, thus taking the lead for this field.
The institute now sells many products related to oxidative stress, and such products are utilized in the fields of medicine, pharmacy, biochemistry, food sciences, etc. The institute also performs assay services utilizing its unique technologies. Preventive medicine and anti-aging are receiving increased attention with the advancement of aging society. In these areas, our Institute will contribute to realizing a healthy, long-lived society by developing and supplying research products.
| Type of marker |
Item | Spec. | Applications | Remark | ||
| Urine | Serum | Cells | ||||
| DNA oxidation marker |
8-OHdG New 8-OHdG Check ELISA (Code. KOG-200SE), ELISA: 450nm, 96 wellsHighly Sensitive 8-OHdG Check ELISA (Code. KOG-HS10E), ELISA: 450nm, 96 wells Anti 8-OHdG monoclonal antibody (N45.1) (Code: MOG-020P / MOG-100P) |
Assay range: 0.5 – 200 ng/mLAssay range: 0.125-10 ng/mL 20 µg / 100 µg of IgG |
OKOK – |
OKOK – |
-OK OK |
Suitable for urine and animal serum. Assay time: 2.5 hours.(KOG-200SE)Suitable for human serum,tissue and cultured cells. Assay time: overnight +1.5 hours(KOG-HS10E) Highly specific to 8-OHdG. Low cross reactivity to oxidized RNA(8-OHGua/8-OHG). |
| Anti Thymidine Glycol (TG) antibody (Code: MTG-100P) Clone 2E8 |
100 µg of IgG | – | – | OK | DNA-specific oxidative stress marker. Specific to TG in DNA polymer. | |
| Lipid oxidation marker |
HEL (13HPODE-Lys adduct) Hexanoyl-Lysine (HEL) ELISA kit (Code: KHL-700E), ELISA: 450nm, 96 wellsAnti Hexanoyl-Lysine (HEL) antibody (Code: MHL-021P) Clone 5F12 |
Assay range: 2 – 700 nmol/L20 µg of IgG |
OK- | OK- | OKOK | Nε-Hexanoyl-Lysine (HEL) is derived from 13-HPODE, and is a novel biomarker for early stage oxidation of n:6 polyunsaturated fatty acid. Detectable in urine, serum, tissue and cultured cells. |
| 4-HNE Anti 4-HNE monoclonal antibody (HNEJ2) (Code: MHN-020P / MHN-100P) |
20 µg / 100 µg of IgG |
– | – | OK | 4-hydroxy-2-nonenal (4-HNE) is one of the major oxidized products of fatty acids. Specific to 4-HNE-modified proteins. | |
| MDA Anti MDA monoclonal antibody (Code: MMD-030n) Clone 1F83Malondialdehyde assay kit (TBARS) (Code: KMD-008W) Colorimetric: 400-700nm scan |
30 µg of IgGAssay range: 0.08 – 10 µmol/L |
-OK | -OK | OKOK | Specific to Malondialdehyde(MDA)-protein adducts such as dihydropyridine-lysine (DHP-lysine). By utilizing 3rd derivative analysis, more reliable results than that by traditional TBARS assay. |
|
| Isoprostane Urinary isoprostane ELISA (Code: KIP-050) ELISA: 450nm, 96wellsSerum isoprostane ELISA (Code: KIP-001W) ELISA: 450nm, 96wells |
Assay range: 0.05 – 100 ng/mLAssay range: 0.05 – 10 ng/mL |
OK- | -OK | -OK | 15-Isoprostane F2t (also known as 8-epi-PGF2α or 8-iso-PGF2α) is produced by peroxidation of lipoproteins. For serum or tissue samples, purification by C18/Silica Sep-Pak will be needed. |
|
| Anti Acrolein (ACR) antibody (Code: MAR-020n / MAR-100n) Clone 5F6 |
20 µg / 100 µg of IgG |
– | – | OK | Specific to ACR modified proteins: formyl-dehydropiperidino-lysine (FDP-lysine). | |
| Anti 4-hydroxy-2-hexenal (4-HHE) antibody (Code: MHH-030n) Clone HHE53 |
30 µg of IgG | – | – | OK | Specific to HHE-histidine Michael adduct (HHE-His). | |
| Anti Crotonaldehyde (CRA) antibody (Code: MCA-030n) Clone 82D3 |
30 µg of IgG | – | – | OK | Specific to CRA-protein aduucts. | |
| Anti Methylglyoxal (MG) antibody (Code: MMG-030n) Clone 3C |
30 µg of IgG | – | – | OK | Specific to MG-protein adduct (argpyrimidine). | |
| Anti 7-ketocholesterol (7-KC) antibody (Code: MKC-020n) Clone 35A |
20 µg of IgG | – | – | OK | Biomarker of cholesterol oxidation. Applicable for frozen tissue only. | |
| Mitochondria Lipid peroxide probe (mitochondria) [NEW] (Code: PLP-466J) |
5 µg x3 | – | – | OK | Fluorescence probe for lipid peroxides specific to mitochondia. Suitable for cultured cells. | |
| Lipid peroxide probe (DPPP) [NEW] (Code: PLP-350J) |
10 mg | – | OK | OK | Fluorescence probe for lipid peroxides. For HPLC application. | |
| Protein oxidation marker |
Dityrosine (Tyr dimer) Dityrosine (DT) ELISA [NEW] (Code: KDT-010E), ELISA: 450nm, 96 wellsAnti Dityrosine (DT) antibody (Code: MDT-020P) Clone 1C3 |
Assay range: 0.05-12 µmol/L20 µg of IgG |
OK- | OK- | -OK | New biomarker specific for protein oxidation. Assay time: overnight + 1.5hours. |
| Anti Dibromo-Tyrosine (DiBrY) antibody (Code: MBY-020P) Clone 3A5 |
20 µg of IgG | – | – | OK | Inflammation-related protein oxidation marker. | |
| NO-modification Anti Nitrotryptophan (NW) antibody [NEW] (Code: MNW-020P) Clone 117C |
20 µg of IgG | – | – | OK | A novel nitrosative stress marker derived from peroxynitrite and tryptophan. | |
| Nitrotyrosine ELISA (Code: KNT-002W) 96 wells |
2 – 1500 nmol/L | – | OK | Δ | ||
| Anti- oxidant assay |
Total Antioxidant Test kit for Potential Antioxidant(PAO), 96 wells (Code: KPA-050) Colorimetric: 480-492 nmTAC Peroxyl assay, 96 wells (Code: KPR-005W) Luminescence |
Assay range: 21.9- 4378 µmol/LAssay range: 0.5～30 µmol/mL |
— | OKOK | OKOK | Total antioxidant assay for serum, foods and beverages. Can detect both hydrophobic and hydrophilic antioxidants. KPR-005W can detect peroxyl radicals(ROO·) scavenging activity. |
| GSH Total Glutathione assay kit 100 tests [NEW] (Code: KGS-419J) Colorimetric: 405-415 nm |
Assay range: 5-100 µmol/L |
– | – | OK | The most abundant antioxidant in living cells. Applicable to a variety of samples such as plant tissues and bacteria. | |
| Enzyme assay |
SOD SOD activity assay kit (WST-1 method) [NEW] (Code: KSD-311J) Colorimetric: 450 nm, 500 testsSOD activity assay kit (Hematoxylin method) (Code: KSD-005W) Colorimetric: 560 nm, 96 wells Cu/Zn-SOD ELISA (Human SOD1) (Code: KSE-040W) ELISA: 450 nm, 96 wells Mn-SOD ELISA (Human SOD2) (Code: KSG-025W) ELISA: 450 nm, 96 wells ec-SOD ELISA (Human SOD3) (Code: KSF-002W) ELISA: 450 nm, 96 wells |
Assay range: 5-60 U/mL Assay range: 0.04 – 5 ng/mL Assay range: 25 – ng/mL Assay range: 2 – 168 ng/mL |
— – – – |
OKEryth- rocyte OK – – |
OKOK OK OK OK |
Can be applied to all animals, plants and bacteria.SOD1 is mainly located in erythrocytes and tissue cells. Trace concentration of SOD1 would be detected in serum. Mn-SOD(SOD2) is detectable in Mitochondria. |
| CAT Catalase activity assay kit 96 wells (Code: KCA-003W) UV: 240 nm |
Assay range: 6-150 U/mL |
– | Eryth- rocyte |
OK | ||
| MPO activity assay kit (Code: KMP-008W) Colorimetric: 412 nm 96 microplate assays or 30 cubette assays |
Assay range: 8- 400 U/mL |
– | OK | OK | ||
| GSH metabolism Glutathion Peroxidase (GPx) assay kit 192 wells (Code: KGX-005W) Colorimetric: 340 nmGlutathion Reductase (GR) assay kit 192 wells (Code: KGR-015W) Colorimetric: 340 nm |
Assay range: 5-60 mU/mLAssay range: 1.5-20 mU/mL |
— | BloodBlood | OKOK | ||
| Redox regulation markers |
Thioredoxin Thioredoxin I ELISA (human) 96 wells (Code: KRX-004W) ELISA: 450 nm |
Assay range: 0.4- 25 ng/mL |
– | OK | Δ | |
| Thioredoxin reductase I ELISA (human) 96 wells (Code: KRR-004W) ELISA: 450 nm |
Assay range: 0.4- 25 ng/mL |
– | OK | Δ | ||
| Peroxiredoxin I ELISA (human) 96 wells (Code: KPX-005W) ELISA: 450 nm |
Assay range: 0.5 – 32 ng/mL |
– | OK | Δ | ||
| Peroxiredoxin III ELISA (human) 96 wells (Code: KPZ-002W) ELISA: 450 nm |
Assay range: 2 – 32 ng/mL |
– | OK | Δ | ||
| Trace elements assay |
Fe Iron assay kit (Ferrozine) 100 tests (Code: CFE-005) Colorimetric: 540-580 nmIron assay kit (Nitroso-PSAP) 100 tests (Code: CFE-010) Colorimetric: 740-760 nm UIBC assay kit (Bathophenanthroline) 100 tests (Code: CBC-800) Colorimetric: 540-550 nm |
Assay range: 5-1,000 µg/dLAssay range: 10-1,000 µg/dL Assay range: 10-800 µg/dL |
OKOK – |
OKOK OK |
OKOK – |
Iron (Fe) is an important pro-oxidant factor which produce hydroxyl radical by fenton reaction. CFE-005 and CFE-010 can be also applied to extractes from animal tissue, plants and hair samples.Detection of unsaturated iron binding capacity(UIBC). Species independent. |
| Cu Copper assay kit (3,5-DiBr-PAESA) 100 tests (Code: CCU-400) Colorimetric: 570-590 nm |
Assay range: 3-400 µg/dL |
OK | OK | OK | Can detect total (free and ceruloplasmin – bond) copper. Also applicable to extractes from tissues, plants and hair. | |
| Zn Zink assay kit (5-Br-PAPS) (Code: CZN-001) Colorimetric: 550-580 nm |
Assay range: 4-1,000 µg/dL |
OK | OK | OK | Can detect total (free and protein – bond) zinc. Also applicable to extractes from tissues, plants and hair. | |
| Ca Calcium assay kit (Chlorophosphonazo III) (Code: CCA-020) Colorimetric: 680-700 nm, 200 testsCalcium assay kit (o-Cresolphtalein-Complexone) (Code: CCA-030) Colorimetric: 560-590 nm, 200 tests |
Assay range: 0.05-20 mg/dLAssay range: 0.2-30 mg/dL |
OKOK | OKOK | OKOK | Applicable to serum, plasma, urine, saliva, extracts from tissues, plants, hair and bone samples. Avoid EDTA containing samples. | |
| Mg Magnesium assay kit (Xyridil Blue-I) 100 tests (Code: CMG-035) Colorimetric: 660 nm |
Assay range: 0.05-3.5 mg/dL |
OK | OK | OK | Applicable to serum, plasma, urine, saliva, extracts from tissues, plants and hair. May be interfared by EDTA, haemolysis, anticoagulants and potassium. | |